P21 Polyclonal Antibody
Catalogue Number: BS-55160R-BSS
BS-55160R Image
BS-55160R Image
BS-55160R Image
BS-55160R Image
BS-55160R Image
BS-55160R Image
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:100 overnight at 4°C, DAB staining.
BS-55160R Image
Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:100 overnight at 4°C, DAB staining.
BS-55160R Image
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:100 overnight at 4°C, DAB staining.
BS-55160R Image
Lane 1: Hela cell lysates; Lane 2: P21 knockout (KO) HeLa cell lysates probed with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55160R Image
Lane 1: U87-MG cell lysates probed with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CAP20;CDKN1;CIP1;MDA-6;P21;SDI1;WAF1;p21CIP1;CDKN1A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human P21 (NP_001207707.1). |
| Application: | ELISA, IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Additional Text
Gene ID
1026
Gene Name
CDKN1A
Uniprot ID
P38936
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bs-55160R Datasheet
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