SIRT3 Polyclonal Antibody
Catalogue Number: BS-55199R-BSS
BS-55199R Image
BS-55199R Image
BS-55199R Image
BS-55199R Image
BS-55199R Image
BS-55199R Image
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4°C, DAB staining.
BS-55199R Image
Paraformaldehyde-fixed, paraffin embedded Human thyroid cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4°C, DAB staining.
BS-55199R Image
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4°C, DAB staining.
BS-55199R Image
Lane 1: SW620 cell lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55199R Image
Lane 1: Hela cell lysates; Lane 2: SIRT3 knockout (KO) HeLa cell lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SIRT3;SIR2L3;sirtuin 3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1). |
| Application: | ELISA, IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Additional Text
Gene ID
23410
Uniprot ID
Q9NTG7
Gene Name
SIRT3
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bs-55199R Datasheet
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