SQSTM1/p62 Polyclonal Antibody
Catalogue Number: BS-55207R-BSS
BS-55207R Image
BS-55207R Image
BS-55207R Image
BS-55207R Image
BS-55207R Image
BS-55207R Image
BS-55207R Image
BS-55207R Image
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SQSTM1/p62) polyclonal Antibody, Unconjugated (bs-55207R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BS-55207R Image
Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1/p62 Polyclonal Antibody, Unconjugated (bs-55207R) at 1:100 overnight at 4°C, DAB staining.
BS-55207R Image
Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1/p62 Polyclonal Antibody, Unconjugated (bs-55207R) at 1:100 overnight at 4°C, DAB staining.
BS-55207R Image
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1/p62 Polyclonal Antibody, Unconjugated (bs-55207R) at 1:100 overnight at 4°C, DAB staining.
BS-55207R Image
Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1/p62 Polyclonal Antibody, Unconjugated (bs-55207R) at 1:100 overnight at 4°C, DAB staining.
BS-55207R Image
Lane 1: 293T cell lysates; Lane 2: SQSTM1/p62 knockout (KO) 293T cell lysates probed with SQSTM1/p62 Polyclonal Antibody, Unconjugated (bs-55207R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55207R Image
Lane 1: 293T cell lysates; Lane 2: Hela cell lysates; Lane 3: U87-MG cell lysates probed with SQSTM1/p62 Polyclonal Antibody, Unconjugated (bs-55207R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human SQSTM1/p62 |
| Application: | ELISA, ICC, IF, IHC-P, WB |
Description
Description: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
Additional Text
Gene Name
SQSTM1
Uniprot ID
Q13501
Gene ID
8878
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
A170; EBI 3 associated protein of 60 kDa; EBI3 associated protein of 60 kDa; EBI3-associated protein of 60 kDa; EBIAP; MGC127197; ORCA; OSF-6; Osi; OSIL; Oxidative stress induced like; p60; p62; p62B; Paget disease of bone 3; PDB 3; PDB3; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; Phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome 1; Sequestosome-1; SQSTM 1; SQSTM_HUMAN; Sqstm1; STAP; STONE14; Ubiquitin binding protein p62; Ubiquitin-binding protein p62; ZIP 3; ZIP; ZIP3
Storage Note
Store at -20°C for 12 months.
bs-55207R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations