PDK1 (Ser-241), Phosphospecific Antibody
Catalogue Number: BS-70602R-BSS
| Manufacturer: | Bioss Inc |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | PDPK1, PKBK |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Phospho-PDK1 (Ser-241) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 241 in human PDK1. This sequence is highly conserved in rat and mouse PDK1. |
| Application: | IHC-P, WB, IHC |
Description
Description: 3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 is important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.
Additional Text
Gene ID
5170
Gene Name
PDPK1
Uniprot ID
O15530
Purification
Affinity Purified
Modification
Phosphorylated, Ser241
Antibody Clonality
Polyclonal
Storage Note
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
BS-70602R Datasheet
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