NGFR Polyclonal Antibody
Catalogue Number: BS-7122R-BSS
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Mouse spleen cells were probed with NGFR Polyclonal Antibody (bs-7122R) for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Lane 1: Mouse Cerebellum; Probed with NGFR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:300, overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin-embedded Rat Liver tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with NGFR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
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Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with NGFR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:200 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
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Mouse Spleen Cells were fixed with ice-cold 70% methanol (Overnight at 4°C). The cells were then incubated in 1X PBS / 2% BSA / 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with NGFR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:100 for 30 minutes at room temp, followed by incubation with a Goat anti-rabbit IgG-FITC secondary antibody. The graph compares the primary antibody staining (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).
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Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human NGFR/p75NTR |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis.
Additional Text
Gene Name
NGFR
Gene ID
4804
Uniprot ID
P08138
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
Gp80 LNGFR; ngfrp75; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; NGF receptor; p75 ICD; CD271; CD271 antigen; Gp80 LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor; Nerve growth factor receptor TNFR superfamily member 16; NGF receptor; NGFR; p75 ICD; p75 Neurotrophin receptor; p75 NTR; TNFR Superfamily Member 16; TNFRSF16; Tumor necrosis factor receptor superfamily member 16; TNR16_HUMAN.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-7122R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations