STAT3 (3F5) Monoclonal Antibody
Catalogue Number: BSM-33218M-BSS
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293T Cell lysates; probed with STAT3 (3F5) Monoclonal Antibody, unconjugated (bsm-33218M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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A594 Cell lysates; probed with STAT3 (3F5) Monoclonal Antibody, unconjugated (bsm-33218M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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HepG2 Cell lysates; probed with STAT3 (3F5) Monoclonal Antibody, unconjugated (bsm-33218M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:2000 overnight at 4°C, DAB staining.
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Lane 1: Human Siha cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33218M ) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: STAT3 knockout (KO) A549 cell lysates; Lane 2: Human A549 cell (Control) lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human THP-1 cell lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Signal transducer and activator of transcription 3; STAT3; Acute-phase response factor; APRF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Mouse |
| Clone: | 3F5 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | IHC-P, WB |
Description
Description: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genesPubMed:12359225. Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476).
Additional Text
Gene Name
STAT3
Gene ID
6774
Uniprot ID
P40763
Purification
Protein G purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-33218M Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations