STAT3 (5H4) Monoclonal antibody
Catalogue Number: BSM-33223M-BSS
BSM-33223M Image
BSM-33223M Image
BSM-33223M Image
BSM-33223M Image
bsm-33223M Image
BSM-33223M Image
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4°C, DAB staining.
BSM-33223M Image
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4°C, DAB staining.
BSM-33223M Image
Lane 1: STAT3 knockout (KO) A549 cell lysates; Lane 2: Human A549 cell (Control) lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-33223M Image
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human THP-1 cell lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-33223M Image
Lane 1: Mouse Heart lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Lung lysates probed with STAT3 Polyclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 5H4 |
| Isotype: | IgG |
| Immunogen: | Recombinant human STAT3 Protein |
| Application: | IHC-P, WB |
Description
Description: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063.
Additional Text
Gene Name
STAT3
Gene ID
6774
Uniprot ID
P40763
Purification
Protein G purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Specificity
1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3 (acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-33223M Datasheet
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