GFAP Monoclonal Antibody

Catalogue Number: BSM-42001M-BSS

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-42001M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-42001M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

BSM-42001M Image

Lane 1: Human U87MG cell lysates lysates; Lane 2: Human U251 cell lysates probed with GFAP Monoclonal Antibody, Unconjugated (bsm-42001M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-42001M Image

Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates probed with GFAP Monoclonal Antibody, Unconjugated (bsm-42001M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

BSM-42001M Image

Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

BSM-42001M Image

25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (bsm-42001M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	Astrocyte Marker; FLJ45472; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	8H10
Isotype:	IgG
Immunogen:	Recombinant human GFAP protein
Application:	IF, IHC-P, WB, IHC-Fr

Overview
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Description

Description: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.