UCHL1 (3C2) Monoclonal Antibody
Catalogue Number: BSM-51100M-BSS
BSM-51100M Image
BSM-51100M Image
BSM-51100M Image
Lane 1: U266 Cell lysates; Lane 2: Neuro-2a Cell lysates; Lane 3: PC-12 Cell lysates; Lane 4: C6 Cell lysates; Lane 5: mouse brain lysates; probed with UCHL1 (346CT2.2.1) Monoclonal Antibody, unconjugated (bsm-51100M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-51100M Image
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with UCHL1 (346CT2.2.1) Monoclonal Antibody (bsm-51100M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Preservative: | 0.09% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; UCHL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 3C2 |
| Isotype: | IgG1 |
| Immunogen: | This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein. |
| Application: | IF, IHC-P, WB |
Description
Description: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Additional Text
Gene ID
7345
Gene Name
UCHL1
Uniprot ID
P09936
Purification
Protein G purified
Concentration
0.5 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-51100M Datasheet
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