ADAM17 (9E7) Monoclonal Antibody
Catalogue Number: BSM-52009R-BSS
BSM-52009R Image
BSM-52009R Image
BSM-52009R Image
BSM-52009R Image
BSM-52009R Image
BSM-52009R Image
bsm-52009R Image
BSM-52009R Image
SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4°C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.
BSM-52009R Image
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52009R Image
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52009R Image
Flow cytometric analysis of Hela cells with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
BSM-52009R Image
Lane 1: Pancreas lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52009R Image
Lane 1: Mouse Spleen lysates; Lane 2: Jurkat cell lysates; Lane 3: SW480 cell lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-52009R Image
Jurkat cell lysate probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human ADAM17 protein, around 700-824aa. |
| Application: | FC, ICC, IF, WB |
Description
Description: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.
Additional Text
Gene Name
ADAM17
Uniprot ID
P78536
Gene ID
6868
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Specificity
Disintegrin and metalloproteinase domain-containing protein 17; ADAM metallopeptidase domain 17; CSVP; TACE; NISBD; CD156B; NISBD1; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; Tumor Necrosis Factor Alpha Converting Enzyme.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52009R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations