A-RAF Recombinant Antibody
Catalogue Number: BSM-52015R-BSS
BSM-52015R Image
BSM-52015R Image
BSM-52015R Image
BSM-52015R Image
BSM-52015R Image
BSM-52015R Image
Paraformaldehyde-fixed, paraffin embedded human kidney section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52015R Image
NIH/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52015R Image
HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52015R Image
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates probed with A-RAF Monoclonal Antibody, Unconjugated (bsm-52015R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52015R Image
HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:1000 overnight at 4°C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | 24D1 |
| Isotype: | IgG |
| Immunogen: | Recombinant human A-RAF protein (150-300aa) |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Additional Text
Gene ID
369
Gene Name
ARAF
Uniprot ID
P10398
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
A raf 1; A-Raf; Raf1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1(murine sarcoma 3611 virus).
bsm-52015R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations