c-Jun (4A11) Monoclonal Antibody
Catalogue Number: BSM-52035R-BSS
BSM-52035R Image
BSM-52035R Image
BSM-52035R Image
BSM-52035R Image
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52035R Image
NIH/3T3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52035R Image
Paraformaldehyde-fixed, paraffin embedded human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | AP1; AP-1; c-Jun; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 4A11 |
| Isotype: | IgG |
| Immunogen: | Recombinant human c-Jun protein, around N-terminal 100aa. |
| Application: | WB |
Description
Description: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Additional Text
Gene ID
3725
Gene Name
JUN
Uniprot ID
P05412
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
BSM-52035R Datasheet
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