Cyclin B1 (2E6) Monoclonal Antibody
Catalogue Number: BSM-52044R-BSS
BSM-52044R Image
BSM-52044R Image
BSM-52044R Image
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52044R Image
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: HeLa, 3: HT-29 Protein loading quantity: 20 µg Exposure time: 60 s Predicted MW: 55 kDa Observed MW: 55 kDa
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | G2/mitotic-specific cyclin-B1; CCNB1; CCNB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human Cyclin B1 protein, around N-terminal 150aa. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Additional Text
Gene ID
891
Gene Name
CCNB1
Uniprot ID
P14635
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52044R Datasheet
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