Cyclin D3 (4A8) Monoclonal Antibody
Catalogue Number: BSM-52047R-BSS
BSM-52047R Image
BSM-52047R Image
bsm-52047R Image
BSM-52047R Image
A431 cells were stained with Cyclin D3 (4A8) Monoclonal Antibody (bsm-52047R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52047R Image
Lane 1: Human MOLT-4 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Panc-1 cell lysates probed with Cyclin D3 Monoclonal Antibody, Unconjugated (bsm-52047R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-52047R Image
Lane 1: Jurkat; Lane 2: Hela lysates probed with Cyclin D3 (4A8) Monoclonal Antibody (bsm-52047R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | G1/S-specific cyclin-D3; CCND3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 4A8 |
| Isotype: | IgG |
| Immunogen: | Recombinant human Cyclin D3 protein, around C-terminal 200aa. |
| Application: | ICC, IF, WB |
Description
Description: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Additional Text
Gene ID
896
Gene Name
CCND3
Uniprot ID
P30281
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52047R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations