Cyclin E1 (4H7) Monoclonal Antibody
Catalogue Number: BSM-52048R-BSS
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
BSM-52048R Image
HepG2 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52048R Image
MCF-7 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Human laryngeal cancer tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Human laryngeal cancer tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52048R Image
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
BSM-52048R Image
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
BSM-52048R Image
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
BSM-52048R Image
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclin E1 (4H7) Monoclonal Antibody, Unconjugated (bsm-52048R) at 1:200 overnight at 4°C, DAB staining.
BSM-52048R Image
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin E1) monoclonal Antibody, Unconjugated (bsm-52048R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Cyclin E1 phospho T77; p-Cyclin E1 phospho T77; Cyclin E1 Thr77; CCNE 1; CCNE;CCNE1;Cyclin E1 antibody Cyclin Es;Cyclin Et;CyclinE; G1/S specic cyclin E. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 4H7 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide derive from human cyclin e1 |
| Application: | ICC, IF, IHC-P |
Description
Description: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Additional Text
Gene ID
898
Gene Name
CCNE1
Uniprot ID
P24864
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52048R Datasheet
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