HDAC2 (3B7) Monoclonal Antibody

Catalogue Number: BSM-52082R-BSS

BSM-52082R Image

bsm-52082R Image

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

SHG-44 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded mouse spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

BSM-52082R Image

HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

BSM-52082R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52082R Image

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52082R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52082R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse intestine tissue incubated with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52082R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse spinal cord tissue incubated with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Mouse spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Lane 1: Mouse Lung lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Large intestine lysates; Lane 4: NIH'3T3 cell lysates ; Lane 5: 293T cell lysates probed with HDAC2 Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

BSM-52082R Image

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody, Unconjugated (bsm-52082R) at 1:200 overnight at 4°C, DAB staining.

bsm-52082R Image

Lane 1: 293T Cell lysates; Lane 2: K562 lysates; probed with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Ph:	7.4
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	HD2; RPD3; YAF1; Histone deacetylase 2; HDAC2
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Rabbit
Clone:	3B7
Isotype:	IgG
Immunogen:	Recombinant human HDAC2 protein, around C-terminal 150aa.
Application:	ICC, IF, IHC-P, WB

Overview
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Description

Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.