HDAC6 (6B4) Monoclonal Antibody
Catalogue Number: BSM-52086R-BSS
BSM-52086R Image
BSM-52086R Image
BSM-52086R Image
BSM-52086R Image
BSM-52086R Image
BSM-52086R Image
293 cells were stained with HDAC6 (6B4) Monoclonal Antibody (bsm-52086R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52086R Image
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with HDAC6 (6B4) Monoclonal Antibody (bsm-52086R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52086R Image
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: COS-7, 3: K562 Protein loading quantity: 20 µg Exposure time: 60 s Predicted MW: 131 kDa Observed MW: 160 kDa
BSM-52086R Image
Tissue: Human kidney Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52086R
BSM-52086R Image
Tissue: Human testis Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52086R
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | HD6; JM21; CPBHM; PPP1R90; Histone deacetylase 6; HDAC6; KIAA0901 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 6b4 |
| Isotype: | IgG |
| Immunogen: | Recombinant human HDAC6 protein, around C-terminal 150aa. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.
Additional Text
Gene Name
HDAC6
Uniprot ID
Q9UBN7
Gene ID
10013
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
BSM-52086R Datasheet
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