H2AX Monoclonal Antibody
Catalogue Number: BSM-52163R-BSS
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
BSM-52163R Image
Lane 1: Etoposide treated HepG2 lysates; Lane 2: Untreated HepG2 lysates probed with Histone H2A.X(S139) (1C14) Monoclonal Antibody (bsm-52163R) at 1:1000.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, (bsn-52163R) at 1:1000 overnight at 4°C, followed by a conjugated secondary and DAB staining
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4°C, DAB staining.
BSM-52163R Image
Lane 1: Human MCF-7 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Hela cell lysates probed with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52163R Image
Lane 1: Human 293T-UV cell lysates; Lane 2: Human HeLa-UV cell lysates; Lane 3: Human Jurkat-UV cell lysates probed with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | H2A histone family member X; Histone H2A.x; H2afx; H2a.x; H2ax; Hist5-2ax; H2A.X; H2A/X; H2AX; H2AFX; H2AX_HUMAN. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 1C14 |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser139:QA(p-S)QE. |
| Application: | IHC-P, WB |
Description
Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008].
Additional Text
Gene ID
3014
Uniprot ID
P16104
Modification
Phosphorylated, Ser139
Purification
Protein A purified
Gene Name
H2ax
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52163R Datasheet
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