JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody
Catalogue Number: BSM-52171R-BSS
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
BSM-52171R Image
HeLa cells were stained with JAK2(Y1007+Y1008) (6E5 ) Monoclonal Antibody (bsm-52171R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52171R Image
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody (bsm-52171R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52171R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody (bsm-52171R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52171R Image
Paraformaldehyde-fixed and paraffin-embedded Rat spleen tissue incubated with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody (bsm-52171R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52171R Image
Paraformaldehyde-fixed and paraffin-embedded Rat lung tissue incubated with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody (bsm-52171R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52171R Image
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody (bsm-52171R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52171R Image
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody, Unconjugated (bsm-52171R) at 1:200 overnight at 4°C, DAB staining.
BSM-52171R Image
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody, Unconjugated (bsm-52171R) at 1:200 overnight at 4°C, DAB staining.
BSM-52171R Image
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody, Unconjugated (bsm-52171R) at 1:200 overnight at 4°C, DAB staining.
BSM-52171R Image
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody, Unconjugated (bsm-52171R) at 1:200 overnight at 4°C, DAB staining.
BSM-52171R Image
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with JAK2(Y1007+Y1008) (6E5) Monoclonal Antibody, Unconjugated (bsm-52171R) at 1:200 overnight at 4°C, DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | JTK1; THCYT3; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2; JAK2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide derived from human JAK2(Y1007+Y1008), around 1000-1050aa (phospho Y1007+Y1008). |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Additional Text
Gene Name
JAK2
Gene ID
3717
Uniprot ID
O60674
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Phosphorylated, Tyr1007
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52171R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations