Nrf2(S40) (7G4) Monoclonal Antibody

Catalogue Number: BSM-52179R-BSS

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bsm-52179R Image

BSM-52179R Image

HepG2 cells were stained with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

BSM-52179R Image

A549 cells were stained with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody, Unconjugated (bsm-52179R) at 1:200 overnight at 4°C, DAB staining.

BSM-52179R Image

THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nrf2(S40) (7G4) Monoclonal Antibody(bsm-52179R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) monoclonal Antibody, Unconjugated (bsm-52179R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Lane 1: HepG2; Lane 2: Raji lysates probed with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Ph:	7.4
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2; NFE2L2
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Rabbit
Clone:	7G4
Isotype:	IgG
Immunogen:	Synthetic peptide derived from human Nrf2(S40), around 1-70aa (phospho S40).
Application:	FC, ICC, IF, IHC-P, WB

Overview
Datasheets

Description

Description: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.