Smad1 (2D9) Monoclonal Antibody
Catalogue Number: BSM-52222R-BSS
BSM-52222R Image
BSM-52222R Image
bsm-52222R Image
BSM-52222R Image
MCF-7 cells were stained with Smad1 (2D9) Monoclonal Antibody (bsm-52222R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52222R Image
Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MCF-7 cell lysates probed with Smad1 Monoclonal Antibody, Unconjugated (bsm-52222R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-52222R Image
Lane 1: Human skeletal muscle; Lane 2: Hela; probed with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 2D9 |
| Isotype: | IgG |
| Immunogen: | Recombinant human Smad1 protein, around N-terminal 1-200aa. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].
Additional Text
Gene Name
SMAD1
Gene ID
4086
Uniprot ID
Q15797
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Specificity
Smad1 phospho S465; Smad1 phospho Ser465; p-Smad1 Ser465; Mothers against decapentaplegic homolog 1; BSP 1; BSP1; BSP-1; Dwarfin A; DwfA; hSMAD 1; hSMAD1; JV 41; JV4 1; JV4-1; JV41; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; MADH 1; MADH1; Madr 1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; Sma and Mad related protein 1; SMAD 1; SMAD-1; SMAD family member 1; SMAD mothers against DPP homolog 1 Drosophila; Spinal muscular atrophy distal with upper limb predominance; TGF beta signaling protein 1; Transforming growth factor beta signaling protein 1; SMAD1_HUMAN; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1; Smad1; Transforming growth factor-beta-signaling protein 1.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52222R Datasheet
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