Smad3 (3D1) Monoclonal Antibody
Catalogue Number: BSM-52224R-BSS
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
BSM-52224R Image
bsm-52224R Image
BSM-52224R Image
SW480 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
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Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52224R Image
Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Flow cytometric analysis of Hela cells with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Large intestine lysates; Lane 4: Mouse Ovary lysates ; Lane 5: Mouse Lung lysates probed with Smad3 Monoclonal Antibody, Unconjugated (bsm-52224R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52224R Image
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody, Unconjugated (bsm-52224R) at 1:200 overnight at 4°C, DAB staining.
BSM-52224R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody, Unconjugated (bsm-52224R) at 1:200 overnight at 4°C, DAB staining.
BSM-52224R Image
Paraformaldehyde-fixed, paraffin embedded Mouse ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody, Unconjugated (bsm-52224R) at 1:200 overnight at 4°C, DAB staining.
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HUVEC cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (3D1) Monoclonal Antibody(bsm-52224R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Raw264.7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Smad3) monoclonal Antibody, Unconjugated (bsm-52224R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Lane 1: A549 Cell lysates; probed with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 3D1 |
| Isotype: | IgG |
| Immunogen: | Recombinant human Smad3 protein, around N-terminal 1-200aa. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.
Additional Text
Gene ID
4088
Gene Name
SMAD3
Uniprot ID
P84022
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Specificity
Smad3 phospho Ser423; Smad3 phospho S423; p-Smad3 Ser423; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52224R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations