Stat3 (6G5) Monoclonal Antibody
Catalogue Number: BSM-52235R-BSS
BSM-52235R Image
BSM-52235R Image
BSM-52235R Image
BSM-52235R Image
bsm-52235R Image
BSM-52235R Image
CRC cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52235R Image
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52235R Image
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Stat3 Antibody(bsm-52235R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BSM-52235R Image
Lane 1: Hela cell lysates; Lane 2: A431 cell lysates probed with Stat3 Monoclonal Antibody, Unconjugated (bsm-52235R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-52235R Image
Lane 1: HeLa lysates; Lane 2: SHG-44 lysates; Lane 3: SW480 Lysates; probed with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | APRF; HIES; ADMIO; Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 6G5 |
| Isotype: | IgG |
| Immunogen: | Recombinant human Stat3 protein, around N-terminal 1-100aa. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).
Additional Text
Gene Name
STAT3
Gene ID
6774
Uniprot ID
P40763
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52235R Datasheet
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