STAT6 (9A7) Monoclonal Antibody
Catalogue Number: BSM-52239R-BSS
BSM-52239R Image
BSM-52239R Image
BSM-52239R Image
BSM-52239R Image
BSM-52239R Image
bsm-52239R Image
BSM-52239R Image
Hela cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52239R Image
HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52239R Image
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with STAT6 (9A7) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52239R Image
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with STAT6 Antibody(bsm-52239R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BSM-52239R Image
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human Hela cell lysates probed with STAT6 monoclonal Antibody, Unconjugated (bsm-52239R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-52239R Image
Lane 1: NIH/3T3 lysates; Lane 2: Hela lysates probed with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | STAT6B; STAT6C; D12S1644; IL-4-STAT; Signal transducer and activator of transcription 6; IL-4 Stat; STAT6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 9A7 |
| Isotype: | IgG |
| Immunogen: | Recombinant human STAT6 protein, around 700-800aa. |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Additional Text
Gene Name
STAT6
Gene ID
6778
Uniprot ID
P42226
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52239R Datasheet
SUPPORT
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DELIVERY
we offer free delivery to UK universities and non profit organisations