HMGB1 Recombinant Antibody
Catalogue Number: BSM-52250R-BSS
BSM-52250R Image
BSM-52250R Image
BSM-52250R Image
BSM-52250R Image
BSM-52250R Image
BSM-52250R Image
BSM-52250R Image
BSM-52250R Image
Lane 1: MCF-7; Lane 2: PC12; Lane 3: F9, probed with HMGB1 (5H4 ) Monoclonal Antibody (bsm-52250R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52250R Image
IF(ICC) staining with HMGB1 (5H4) Monoclonal Antibody (bsm-52250R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52250R Image
IF(ICC) staining with HMGB1 (5H4) Monoclonal Antibody (bsm-52250R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52250R Image
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with HMGB1 (5H4) Monoclonal Antibody (bsm-52250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52250R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with HMGB1 (5H4) Monoclonal Antibody (bsm-52250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52250R Image
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with HMGB1 (5H4) Monoclonal Antibody (bsm-52250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52250R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with HMGB1 (5H4) Monoclonal Antibody (bsm-52250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 5H4 |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide derived from human HMGB1 |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).
Additional Text
Gene ID
3146
Gene Name
HMGB1
Uniprot ID
P09429
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.
bsm-52250R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations