VDAC1 Recombinant Antibody

Catalogue Number: BSM-52251R-BSS

BSM-52251R Image

Lane 1: Raji; Lane 2: HepG2; Lane 3: SW480; Probed with VDAC1 (9A7 ) Monoclonal Antibody (bsm-52251R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52251R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52251R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52251R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52251R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52251R Image

Lane 1: Human Raji cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Mouse Heart lysates; Lane 9: Rat Heart lysates; Lane 10: Mouse Liver lysates; Lane 11: Rat Liver lysates; Lane 12: Mouse Kidney lysates; Lane 13: Rat Kidney lysates; Lane 14: Mouse Muscle lysates probed with VDAC1 (9A7 ) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.

BSM-52251R Image

25 ug total protein per lane of various lysates (see on figure) probed with VDAC1 monoclonal antibody, unconjugated (bsm-52251R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC-1, hVDAC1
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Human VDAC1 protein between 1 to 100 amino acids
Application:	IF, IHC-P, WB, IHC

Overview
Datasheets

Description

Description: Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.