Alkaline phosphatase, tissue-nonspecific isozyme Recombinant Antibody

Catalogue Number: BSM-52252R-BSS

BSM-52252R Image

Line 1: Hela; Line 2: HepG2; Line 3: MG-63; Probed with CDT1 (2H5) Monoclonal Antibody (bsm-54038R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52252R Image

Flow cytometric analysis of HeLa cells with Alkaline Phosphatase (1G9 ) Monoclonal Antibody (bsm-52252R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).

BSM-52252R Image

IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52252R Image

IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52252R Image

IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52252R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52252R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52252R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse small intestine tissue incubated with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52252R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52252R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52252R Image

Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: Jurkat Protein loading quantity: 20 µg Exposure time: 60 s Predicted MW: 57 kDa Observed MW: 60 kDa

BSM-52252R Image

25 ug total protein per lane of various lysates (see on figure) probed with Alkaline phosphatase, tissue-nonspecific isozyme monoclonal antibody, unconjugated (bsm-52252R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52252R Image

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase liver/bone/kidney isozyme, AP-TNAP, TNSALP; ALPL
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human Alkaline Phosphatase between 150 to 250 amino acids
Application:	WB