Phospho-Histone H3 (S10) Recombinant Antibody
Catalogue Number: BSM-52261R-BSS
BSM-52261R Image
BSM-52261R Image
BSM-52261R Image
BSM-52261R Image
BSM-52261R Image
BSM-52261R Image
Lane 1: Untreated HeLa cell lysate; Lane 2: HeLa cell lysate treated with calyculin A; Lane 3: HeLa cell lysate treated with calyculin A and alkaline phosphatase; Phospho-Histone H3 (S10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:1000 dilution, Histone H3 antibody and GAPDH antibody as control, incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52261R Image
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52261R Image
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52261R Image
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52261R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | H3 3 like sequence MH921; H3 3A; H3 a; H3 b; H3 c; H3 d; H3 f; H3 h; H3 histone family member E pseudogene; H3 i; H3 j; H3 k; H3 l; H33_HUMAN; H3F3; H3f3b; Histone H3 3 pseudogene; Histone H3.3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 2F6 |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding surrounding Ser10 of human Histone H3 |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Additional Text
Modification
Phosphorylated, Ser10
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Uniprot ID
P68431, P84243
Gene ID
3020; 3021; 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Gene Name
H3-3A, H3-3B, H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8
Storage Note
Store at -20°C for 12 months.
bsm-52261R Datasheet
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DELIVERY
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