GAPDH Recombinant Antibody

Catalogue Number: BSM-52262R-BSS

BSM-52262R Image

Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates; Lane 3: Mouse Spleen lysates; Lane 4: Mouse Testis lysates; Lane 5: Mouse Cerebrum lysates; Lane 6: Rat Heart lysates; Lane 7: Rat Kidney lysates; Lane 8: Rat Spleen lysates; Lane 9: Rat Testis lysates; Lane 10: Rat Cerebrum lysates; Lane 11: Human A549 cell lysates; Lane 12: Human 293T lysates; Lane 13: Human HUVEC lysates; Lane 14: Human Hela lysates probed with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52262R Image

Lane 1: NCCIT; Lane 2: PC12; Lane 3: F9; Probed with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52262R Image

Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

BSM-52262R Image

IF(ICC) staining with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52262R Image

IF(ICC) staining with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52262R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52262R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4°C, DAB staining.

BSM-52262R Image

25 ug total protein per lane of various lysates (see on figure) probed with GAPDH (Loading Control) monoclonal antibody, unconjugated (bsm-52262R) at 1:100000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human Spleen ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human Pancreas ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated(bsm-52262R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52262R Image

25 ug total protein per lane of various lysates (see on figure) probed with GAPDH monoclonal antibody, unconjugated (bsm-52262R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:
Application:	FC, ICC, IF, IHC-P, WB, IHC

Overview
Datasheets

Description

Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.