CD9 Recombinant Antibody

Catalogue Number: BSM-52264R-BSS

BSM-52264R Image

Lane 1: Mouse heart; Lane 2: Jurkat; Probed with CD9 (4C2) Monoclonal Antibody (bsm-52264R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52264R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD9 (4C2) Monoclonal Antibody (bsm-52264R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52264R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CD9 (4C2) Monoclonal Antibody (bsm-52264R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52264R Image

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD9 (4C2) Monoclonal Antibody (bsm-52264R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52264R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with CD9 (4C2) Monoclonal Antibody (bsm-52264R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52264R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD9 (4C2) Monoclonal Antibody (bsm-52264R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52264R Image

Lane 1: Mouse Kidney lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Cerebrum lysates; Lane 5: Human HUVEC cell lysates; Lane 6: Human A431 cell lysates; Lane 7: Human SW480 cell lysates probed with SIRT4 CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, DAB staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, DAB staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, DAB staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, DAB staining.

BSM-52264R Image

25 ug total protein per lane of various lysates (see on figure) probed with CD9 monoclonal antibody, unconjugated (bsm-52264R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Thyroid Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52264R Image

Paraformaldehyde-fixed, paraffin embedded HumanTonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD9 Monoclonal Antibody, Unconjugated(bsm-52264R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, CD_antigen: CD9, MRP-1, Tspan-29
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:
Application:	FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).