Caspase-3 Recombinant Antibody
Catalogue Number: BSM-52289R-BSS
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
BSM-52289R Image
Lane 1: Hela; Lane 2: Jurkat; Lane 3: 293 lysate probed with Caspase-3 (5A3) Monoclonal Antibody (bsm-52289R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52289R Image
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Caspase 3 (5A3) Monoclonal Antibody (bsm-52289R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52289R Image
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Caspase 3 (5A3) Monoclonal Antibody (bsm-52289R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52289R Image
Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52289R
BSM-52289R Image
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated(bsm-52289R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
BSM-52289R Image
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated(bsm-52289R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
BSM-52289R Image
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSMA8 Polyclonal Antibody, Unconjugated (bs-19461R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
BSM-52289R Image
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSMA8 Polyclonal Antibody, Unconjugated (bs-19461R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
BSM-52289R Image
25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 monoclonal antibody, unconjugated (bsm-52289R) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
| Manufacturer: | Bioss Inc |
| Type: | Recombinant Monoclonal |
| Alias: | Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP-3, CPP-32, SCA-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Additional Text
Gene Name
CASP3
Gene ID
836
Uniprot ID
P42574
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52289R Datasheet
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