beta Tubulin Recombinant Antibody

Catalogue Number: BSM-52290R-BSS

BSM-52290R Image

Lane 1: Hela; Lane 2: NIH/3T3; Lane 3: PC12 lysate probed with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52290R Image

Flow cytometric analysis of NIH/3T3 cells with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

BSM-52290R Image

IF(ICC) staining with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52290R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52290R Image

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52290R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52290R Image

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human 293T cell lysates probed with beta Tubulin Monoclonal Antibody, Unconjugated (bsm-52290R) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52290R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Tubulin beta chain, Tubulin beta-5 chain, TUBB
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Human beta Tubulin aa 300 to the C-terminus
Application:	FC, ICC, IF, IHC-P, WB, IHC

Overview
Datasheets

Description

Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.