MMP12 Recombinant Antibody

Catalogue Number: BSM-52292R-BSS

BSM-52292R Image

Lane 1: A549; Lane 2: Hela; Lane 3: MCF-7; Lane 4: THP-1 lysate probed with MMP12 (1A4) Monoclonal Antibody (bsm-52292R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52292R Image

Flow cytometric analysis of A549 cells with MMP12 (1A4) Monoclonal Antibody (bsm-52292R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).

BSM-52292R Image

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MMP12 (1A4) Monoclonal Antibody (bsm-52292R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52292R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with MMP12 (1A4) Monoclonal Antibody (bsm-52292R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52292R Image

Flow cytometric analysis of MMP12 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-52292R, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52292R Image

25 ug total protein per lane of various lysates (see on figure) probed with MMP12 monoclonal antibody, unconjugated (bsm-52292R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52292R Image

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Macrophage metalloelastase, Macrophage elastase, Matrix metalloproteinase-12, MME, ME, hME, MMP-12
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human MMP12 between 450 amino acids to to C-terminus
Application:	FC, IHC-P, WB, IHC

Overview
Datasheets

Description

Description: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.