Bcl-2 Recombinant Antibody

Catalogue Number: BSM-52304R-BSS

BSM-52304R Image

Lane 1: Hela; Lane 2: Jurkat lysate probed with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52304R Image

Flow cytometric analysis of Jurkat cells with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

BSM-52304R Image

IF(ICC) staining with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52304R Image

IF(ICC) staining with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52304R Image

IF(ICC) staining with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52304R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52304R Image

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52304R Image

Lane 1: Jurkat cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bsm-52304R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52304R Image

THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCL2 (5F7) Monoclonal Antibody(bs-52304R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

BSM-52304R Image

Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human THP-1 cell lysates probed with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-52304R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

BSM-52304R Image

Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated(bsm-52304R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52304R Image

25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 monoclonal antibody, unconjugated (bsm-52304R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Apoptosis regulator Bcl-2; BCL2
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human Bcl-2 between 1 to 200 amino acids.
Application:	WB

Overview
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Description

Description: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).