MAP2K1 (7D8) Monoclonal Antibody
Catalogue Number: BSM-52311R-BSS
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BSM-52311R Image
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BSM-52311R Image
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BSM-52311R Image
BSM-52311R Image
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Lane 1: A431; Lane 2: HepG2; Lane 3: Hela lysate probed with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Flow cytometric analysis of HeLa cells with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
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IF(ICC) staining with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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IF(ICC) staining with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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IF(ICC) staining with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
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Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52311R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52311R Image
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52311R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1, MAPK/ERK kinase 1; MAP kinase kinase 1; MAPKK1; MKK1; MEK1; MAP2K1; PRKMK1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 7D8 |
| Isotype: | IgG |
| Immunogen: | Recombinant human MEK1 between 1 to 100 amino acids |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Additional Text
Gene Name
MAP2K1
Gene ID
5604
Uniprot ID
Q02750
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52311R Datasheet
SUPPORT
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DELIVERY
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