Bax Recombinant Antibody

Catalogue Number: BSM-52316R-BSS

BSM-52316R Image

Lane 1: Hela; Lane 2: MCF-7; Lane 3: Human liver lysate robed with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:1000 overnight at 4C followed by a conjugated secondary antibody for 60 minutes at 37C.

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Flow cytometric analysis of HeLa cells with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

BSM-52316R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52316R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52316R Image

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52316R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52316R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52316R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52316R Image

Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Spleen lysates; Lane 5: Mouse Lung lysates; Lane 6: Rat Lung lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Raji cell lysates probed with Bax (3C3) Monoclonal Antibody, Unconjugated (bsm-52316R ) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52316R Image

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-52316R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

BSM-52316R Image

25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bsm-52316R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52316R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BCL2L4
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human Bak between 20 to 150 amino acids
Application:	IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.