Tubulin beta-III Recombinant Antibody

Catalogue Number: BSM-52323R-BSS

BSM-52323R Image

Lane 1: Hela; Lane 2: PC-12; Lane 3: SH-SY-5Y lysate probed with Tubulin beta-III (3B7) Monoclonal Antibody (bsm-52323R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-52323R Image

Flow cytometric analysis of N2A cells with Tubulin beta-III (3B7) Monoclonal Antibody (bsm-52323R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

BSM-52323R Image

IF(ICC) staining with Tubulin beta-III (3B7) Monoclonal Antibody (bsm-52323R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52323R Image

IF(ICC) staining with Tubulin beta-III (3B7) Monoclonal Antibody (bsm-52323R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52323R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Tubulin beta-III (3B7) Monoclonal Antibody (bsm-52323R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52323R Image

SHG-44 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (bsm-52323R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

BSM-52323R Image

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (bsm-52323R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

BSM-52323R Image

Flow cytometric analysis of Tubulin beta-III was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-52323R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

BSM-52323R Image

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human U251 cell lysates; Lane 7: Human U87MG cell lysates probed with Tubulin beta-III Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52323R Image

25 ug total protein per lane of various lysates (see on figure) probed with Tubulin beta-III monoclonal antibody, unconjugated (bsm-52323R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

BSM-52323R Image

Paraformaldehyde-fixed, paraffin embedded Human Thyroid Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human beta III Tubulin between 1 to 100 amino acids
Application:	FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.