PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody
Catalogue Number: BSM-52336R-BSS
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
BSM-52336R Image
Lane 1: Hela; Lane 2: PC12; Lane 3: NIH/3T3 lysate probed with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52336R Image
Flow cytometric analysis of Hela cells with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-52336R Image
IF(ICC) staining with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52336R Image
IF(ICC) staining with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52336R Image
IF(ICC) staining with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52336R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52336R Image
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52336R Image
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52336R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Egl nine homolog 2, Estrogen-induced tag 6, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 1, Prolyl hydroxylase domain-containing protein 1, HIF-PH1, HIF-prolyl hydroxylase 1, HPH-1, PHD1, EGLN2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 7G8 |
| Isotype: | IgG |
| Immunogen: | Recombinant human PHD1/prolyl hydroxylase between 1 to 100 amino acids. |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.
Additional Text
Gene Name
EGLN2
Uniprot ID
Q96KS0
Gene ID
112398
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52336R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations