PCNA Recombinant Antibody

Catalogue Number: BSM-52347R-BSS

BSM-52347R Image

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PCNA (1G3) Antibody(bsm-52347R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Flow cytometric analysis of HeLa lysate probed with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

BSM-52347R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52347R Image

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52347R Image

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52347R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52347R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52347R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52347R Image

Lane 1: Rat Testis Lysate;Lane 2: Mouse Testis Lysate;Lane 3: SiHa Cell Lysate;Lane 4: MCF-7 Cell Lysate;Lane 5: A549 Cell Lysate;Lane 6: U937 Cell Lysate;Lane 7: Mouse NIH/3T3 Cell Lysate;Lane 8: HepG2 Cell Lysate;Lane 9: 293T Cell Lysate;Lane 10: A431 Cell Lysate probed with PCNA Polyclonal Antibody, Unconjugated (bsm-52347R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, DAB staining.

BSM-52347R Image

Lane 1: Hela cell lysates; Lane 2: 293 cell lysates; Lane 3: A431 cell lysates probed with PCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat Testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52347R Image

Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-52347R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	ProlifeRating cell nuclear antigen, Cyclin, PCNA
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human PCNA between 100 to 200 amino acids
Application:	FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.