Hsp90 alpha Recombinant Antibody

Catalogue Number: BSM-52353R-BSS

BSM-52353R Image

IF(ICC) staining with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52353R Image

IF(ICC) staining with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52353R Image

IF(ICC) staining with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52353R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52353R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52353R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52353R Image

COS-1 cell lysates probed with Hsp90 alpha (2A1) Monoclonal Antibody (bsm-52353R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37°C.

BSM-52353R Image

25 ug total protein per lane of various lysates (see on figure) probed with Hsp90 alpha monoclonal antibody, unconjugated (bsm-52353R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52353R Image

Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated(bsm-52353R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Heat shock protein HSP 90-alpha, Heat shock 86 kDa, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38, HSP 86, HSP86, LAP-2, LPS-associated protein 2, HSP90AA1
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Full length protein
Application:	ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.