Lamin B1 Recombinant Antibody

Catalogue Number: BSM-52361R-BSS

BSM-52361R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52361R Image

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52361R Image

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52361R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52361R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52361R Image

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Pancreas tissue lysates; Lane 6: Rat Testis tissue lysates; Lane 7: Human U937 cell lysates; Lane 8: Human Jurkat cell lysates; Lane 9: Human Molt-4 cell lysates; Lane 10: Human Hela cell lysates probed with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52316R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

BSM-52361R Image

Lane 1: Mouse Liver lysates; Lane 2: Mouse Brain lysates probed with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52361R Image

25 ug total protein per lane of various lysates (see on figure) probed with lamin B1 monoclonal antibody, unconjugated (bsm-52361R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52361R Image

Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated(bsm-52361R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Lamin-B1; LMNB1; LMN2; LMNB
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human Lamin B1 between 500 to C-terminus.
Application:	ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.