CD86 Recombinant Antibody
Catalogue Number: BSM-52375R-BSS
BSM-52375R Image
BSM-52375R Image
BSM-52375R Image
BSM-52375R Image
BSM-52375R Image
BSM-52375R Image
BSM-52375R Image
BSM-52375R Image
IF(ICC) staining with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52375R Image
IF(ICC) staining with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52375R Image
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52375R Image
Lane 1: Human Raji cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HUVEC cell lysates probed with CD86 Monoclonal Antibody, Unconjugated (bsm-52375R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52375R Image
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD86) polyclonal Antibody, Unconjugated (bsm-52375R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BSM-52375R Image
Lane 1: Raji cell lysates; Lane 2: Jurkat cell lysates probed with CD86 (4G10) Monoclonal Antibody (bsm-52375R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37°C.
BSM-52375R Image
25 ug total protein per lane of various lysates (see on figure) probed with CD86 monoclonal antibody, unconjugated (bsm-52375R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Alias: | T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD_antigen: CD86, CD86, CD28LG2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human CD86 between 1 to 200 amino acids |
| Application: | WB |
Description
Description: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Additional Text
Gene Name
CD86
Uniprot ID
P42081
Gene ID
942
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52375R Datasheet
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