SUMO-1 (7H5) Monoclonal Antibody
Catalogue Number: BSM-52377R-BSS
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
BSM-52377R Image
Flow cytometric analysis of HeLa lysate probed with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-52377R Image
IF(ICC) staining with SUMO-1 (7H5) Monoclonal Antibody (bsm-52376R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52377R Image
IF(ICC) staining with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52377R Image
IF(ICC) staining with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52377R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse thyroid tissue incubated with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52377R Image
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52377R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52377R Image
Paraformaldehyde-fixed and paraffin-embedded Human thyroid tissue incubated with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52377R Image
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52377R Image
Hela cell lysates probed with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Small ubiquitin-related modifier 1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, SUMO-1, GMP1, Smt3C, SUMO1, SMT3C, SMT3H3, UBL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 7H5 |
| Isotype: | IgG |
| Immunogen: | Human Sumo 1 aa 1-100 |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
Additional Text
Gene Name
SUMO1
Gene ID
7341
Uniprot ID
P63165
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52377R Datasheet
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DELIVERY
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