Notch 1 (1F1) Monoclonal Antibody
Catalogue Number: BSM-52378R-BSS
BSM-52378R Image
BSM-52378R Image
BSM-52378R Image
BSM-52378R Image
BSM-52378R Image
BSM-52378R Image
BSM-52378R Image
BSM-52378R Image
Flow cytometric analysis of HeLa lysate probed with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-52378R Image
IF(ICC) staining with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52378R Image
IF(ICC) staining with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52378R Image
IF(ICC) staining with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52378R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52378R Image
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52378R Image
HepG2 cell lysates probed with Notch 1 (1F1) Monoclonal Antibody (bsm-52378R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1, Notch 1, hN1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 1F1 |
| Isotype: | IgG |
| Immunogen: | Recombinant human Notch1 between 2500 to 2600 amino acids |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Additional Text
Gene Name
NOTCH1
Uniprot ID
P46531
Gene ID
4851
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52378R Datasheet
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