Synaptophysin Recombinant Antibody

Catalogue Number: BSM-52379R-BSS

BSM-52379R Image

IF(ICC) staining with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52379R Image

IF(ICC) staining with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:100 in PC-12 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52379R Image

Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52379R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52379R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptophysin (2B1) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:200 overnight at 4°C, DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptophysin (2B1) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:200 overnight at 4°C, DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptophysin (2B1) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:200 overnight at 4°C, DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptophysin (2B1) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:200 overnight at 4°C, DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptophysin (2B1) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:200 overnight at 4°C, DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptophysin (2B1) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:200 overnight at 4°C, DAB staining.

BSM-52379R Image

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum ; Lane 4: Rat Cerebellum lysates probed with Synaptophysin Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (human pancreas ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYP) Monoclonal Antibody, Unconjugated (bsm-52379R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52379R Image

Lane 1:Human Cerebrum lysates; Lane 2: PC-12 cell lysates probed with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37°C.

BSM-52379R Image

25 ug total protein per lane of various lysates (see on figure) probed with Synaptophysin monoclonal antibody, unconjugated (bsm-52379R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52379R Image

25 ug total protein per lane of various lysates (see on figure) probed with Synaptophysin monoclonal antibody, unconjugated (bsm-52379R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Synaptophysin Monoclonal Antibody, Unconjugated(bsm-52379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Synaptophysin Monoclonal Antibody, Unconjugated(bsm-52379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Synaptophysin Monoclonal Antibody, Unconjugated(bsm-52379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

BSM-52379R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Synaptophysin Monoclonal Antibody, Unconjugated(bsm-52379R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Synaptophysin, Major synaptic vesicle protein p38, SYP
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant human SYP protein 210-313/313 amino acids.
Application:	FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).