alpha smooth muscle Actin Recombinant Antibody

Catalogue Number: BSM-52392R-BSS

BSM-52392R Image

bsm-52392R Image

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with alpha smooth muscle Actin (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with alpha smooth muscle Actin (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with alpha smooth muscle Actin (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse intestine tissue incubated with alpha smooth muscle Actin (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with alpha smooth muscle Actin (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Human Lung tissue incubated with ACTA2 (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with ACTA2 (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ACTA2 (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse Skin tissue incubated with ACTA2 (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with ACTA2 (8C3) Monoclonal Antibody (bsm-52392R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52392R Image

Flow cytometric analysis of Hela cells with ACTA2 (8C3) Monoclonal Antibody (bsm-52392R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

BSM-52392R Image

Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Small intestine lysates; Lane 6: Mouse NIH/3T3 cell lysates; Lane 7: Rat Stomach cell lysates; Lane 8: Rat Uterus cell lysates; Lane 9: Human A549 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human A431 cell lysates probed with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ACTA2 (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, DAB staining.

bsm-52392R Image

Lane 1: A549 cell lysates; Lane 2: Hela cell lysates; Lane 3: NIH/3T3 cell lysates; Lane 4: Mouse heart lysates probed with alpha smooth muscle Actin (8C3) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

BSM-52392R Image

Tissue/cell:HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (alpha smooth muscle Actin) monoclonal Antibody, Unconjugated (bsm-52392R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

BSM-52392R Image

25 ug total protein per lane of various lysates (see on figure) probed with alpha smooth muscle Actin monoclonal antibody, unconjugated (bsm-52392R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

BSM-52392R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated(bsm-52392R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Alias:	Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTA2; ACTSA; alpha smooth muscle Actin
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Recombinant protein of human alpha smooth muscle Actin between 1-200 amino acids.
Application:	ICC, IF, IHC-P, WB, IHC-Fr

Overview
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Description

Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.