MKLP1 Recombinant Antibody
Catalogue Number: BSM-52401R-BSS
BSM-52401R Image
BSM-52401R Image
BSM-52401R Image
BSM-52401R Image
BSM-52401R Image
BSM-52401R Image
IF(ICC) staining with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52401R Image
IF(ICC) staining with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52401R Image
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MKLP1 (7C9) Monoclonal Antibody(bsm-52401R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BSM-52401R Image
Lane 1: Rat Lymph node tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates probed with MKLP1 Monoclonal Antibody, Unconjugated (bsm-52401R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52401R Image
Lane 1: A549 cell lysates; Lane 2: Hela cell lysates probed with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Alias: | Kinesin-like protein KIF23, KIF23; Kinesin-like protein 5; Mitotic kinesin-like protein 1; KNSL5; MKLP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human MKLP1 between 800 to 900 amino acids |
| Application: | FC, ICC, IF, WB |
Description
Description: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
Additional Text
Gene Name
KIF23
Uniprot ID
Q02241
Gene ID
9493
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
bsm-52401R Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations