Neuropilin-1 Recombinant Antibody
Catalogue Number: BSM-52479R-BSS
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
BSM-52479R Image
Flow cytometric analysis of HeLa cells with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R)) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-52479R Image
Lane 1: Mouse heart ; Lane 2: Mouse liver; Lane 3:Mouse kidney; Lane 4 : Human liver; Probed with Neuropilin-1 (49H9)) Monoclonal Antibody (bsm-52479R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52479R Image
IF(ICC) staining with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52479R Image
IF(ICC) staining with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52479R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52479R Image
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52479R Image
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52479R Image
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropilin-1(49H9) ) Monoclonal Antibody, Unconjugated (bsm-52479R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-52479R Image
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropilin-1(49H9) ) Monoclonal Antibody, Unconjugated (bsm-52479R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-52479R Image
Lane 1: Mouse Heart Lysates; Lane 2: Mouse Spinal cord Lysates; Lane 3: Rat Kidney Lysates. Probed with Neuropilin-1 monoclonal Antibody, unconjugated (bsm-52479R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Alias: | Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD_antigen: CD304, NRP1, NRP, VEGF165R |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human Neuropilin 1 between 900 to C-terminus |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Additional Text
Gene Name
NRP1
Uniprot ID
O14786
Gene ID
8829
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
bsm-52479R Datasheet
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