Sodium Potassium ATPase Recombinant Antibody

Catalogue Number: BSM-52485R-BSS

BSM-52485R Image

Flow cytometric analysis of HeLa cells with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

BSM-52485R Image

A549 cells Probed with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

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IF(ICC) staining with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-52485R Image

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52485R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52485R Image

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52485R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Sodium Potassium ATPase (13H5) Monoclonal Antibody (bsm-52485R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sodium Potassium ATPase (13H5) Monoclonal Antibody, Unconjugated (bsm-52485R) at 1:200 overnight at 4°C, DAB staining.

BSM-52485R Image

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Sodium Potassium ATPase) monoclonal Antibody, Unconjugated (bsm-52485R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

BSM-52485R Image

25 ug total protein per lane of various lysates (see on figure) probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (bsm-52485R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52485R Image

25 ug total protein per lane of various lysates (see on figure) probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (bsm-52485R) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

BSM-52485R Image

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-52485R) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

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Manufacturer:	Bioss Inc
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Human Sodium Potassium ATPase between 1 to 100 amino acids
Application:	FC, ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Additional Text

Gene ID

476

Gene Name

ATP1A1

Uniprot ID

P05023

Purification

Protein A purified

Antibody Clonality

Recombinant Monoclonal

Modification

Unmodified

Specificity

Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1, Sodium/potassium-transporting ATPase subunit alpha-4, Sodium pump subunit alpha-4, Na(+)/K(+) ATPase alpha-1 subunit, ATP1A1, ATP1B1, ATP1B, Na(+)/K(+) ATPase alpha-4 subunit, ATP1A4, ATP1AL2