Cyclooxygenase 2 Recombinant Antibody
Catalogue Number: BSM-52502R-BSS
BSM-52502R Image
BSM-52502R Image
BSM-52502R Image
BSM-52502R Image
BSM-52502R Image
Lane 1: A549 Cells; Lane 2:THP-1 Cells; Probed with COX2/Cyclooxygenase 2 (14D5) Monoclonal Antibody (bsm-52502R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C
BSM-52502R Image
IF(ICC) staining with COX2/Cyclooxygenase 2 (14D5) Monoclonal Antibody (bsm-52502R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52502R Image
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX2/Cyclooxygenase 2 (14D5) Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4°C, DAB staining.
BSM-52502R Image
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX2/Cyclooxygenase 2 (14D5) Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4°C, DAB staining.
| Manufacturer: | Bioss Inc |
| Type: | Recombinant Monoclonal |
| Alias: | Prostaglandin G/H synthase 2, Cyclooxygenase-2, PHS II, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2, COX-2, PGH synthase 2, PGHS-2, PTGS2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human COX2/ Cyclooxygenase 2 between 50 to 200 amino acids |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Additional Text
Gene Name
PTGS2
Gene ID
5743
Uniprot ID
P35354
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
bsm-52502R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations